Esterification of chitosan with adipic acid catalyzed by immobilized Candida antarctica lipase
B was carried out in this study. Response surface methodology (RSM) based on a four-factor-
five-level small central composite design (SCCD) was employed to model and analyze the
reaction. A total of 21 experiments representing different combinations of the four reaction
parameters including chitosan concentration (0.01-0.10 g/mL), temperature (35-65◦C), time
(15-240 min), and enzyme amount (5-20 % w/w of total substrate) were generated. A partial cubic equation was fitted to the data with a R2 of 0.9738 showing a high correlation between predicted and experimental values. The enzyme was capable of esterifying chitosan with a maximum yield of 45.3%. Chitosan concentration was found to be the most significant parameter which influenced the ester synthesis. At the optimal conditions of a short reaction time 15 min, 65oC, 0.10 g/mL of chitosan and 18.7% of enzyme, the esterification percentage was 43.5%.
Chaibakhsh, N., Abdul Rahman, M., Jesunathan, K., & Basri, M. (2013). Optimization of biocatalytic synthesis of Chitosan Ester using response surface methodology. Chemistry of Solid Materials, 1(2), 41-55.
MLA
Naz Chaibakhsh; M.B. Abdul Rahman; K. Jesunathan; M Basri. "Optimization of biocatalytic synthesis of Chitosan Ester using response surface methodology". Chemistry of Solid Materials, 1, 2, 2013, 41-55.
HARVARD
Chaibakhsh, N., Abdul Rahman, M., Jesunathan, K., Basri, M. (2013). 'Optimization of biocatalytic synthesis of Chitosan Ester using response surface methodology', Chemistry of Solid Materials, 1(2), pp. 41-55.
VANCOUVER
Chaibakhsh, N., Abdul Rahman, M., Jesunathan, K., Basri, M. Optimization of biocatalytic synthesis of Chitosan Ester using response surface methodology. Chemistry of Solid Materials, 2013; 1(2): 41-55.
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